Ozone therapy has gained attention in the field of dentistry as a potential adjunct treatment for various dental conditions. Ozone, a colorless gas with a characteristic odor, has been shown to have antimicrobial, disinfectant, biocompatibility, and healing properties (Naik et al., 2016). It is being explored as a potential atraumatic, biologically-based treatment for conditions encountered in dental practice (Nogales et al., 2008). Ozone therapy has been used in dentistry for a range of applications, including the prevention and management of dental caries, teeth remineralization, control of infection, disinfection of periodontal pockets, teeth bleaching, management of pain, biofilm removal, enhancement of healing, tissue regeneration, and control of halitosis (Mostafa & Zakaria, 2018).

One of the advantages of ozone therapy is its antimicrobial effect. Ozone has been found to have a rapid microbicidal effect on oral microorganisms in pure culture (Nagayoshi et al., 2004). It has been shown to kill oral microorganisms at a concentration of 2-4 mg/l (Nagayoshi et al., 2004). Ozone has also been found to be effective in disinfecting medical instruments and similar equipment (Baysan et al., 2000). It can be used as a soaking solution for dental instruments (Nagayoshi et al., 2004). Ozone therapy has been proposed as an alternative non-medication therapy for the management of oral lichen planus (Mostafa & Zakaria, 2018). It has also shown success in managing wound healing, gingivitis, periodontitis, osteonecrosis of the jaw, and dentin hypersensitivity (Suh et al., 2019).

In addition to its antimicrobial properties, ozone therapy has been found to have anti-inflammatory effects. Locally applied ozone has been shown to alleviate painful conditions and reduce inflammatory responses (AL-Omiri et al., 2018). Ozone therapy has been explored for its potential to improve the healing of infected wounds, necrotic or poorly oxygenated tissue, and facial nerve regeneration (Özbay et al., 2017). It has also been studied for its potential to enhance the healing of peri-implant mucositis and promote tissue repair in periapical lesions (Butera et al., 2023; Silva et al., 2022).

The use of ozone therapy in dentistry is still evolving, and more research is needed to establish safe and well-defined parameters for its use. Randomized controlled trials are necessary to determine the precise indications and guidelines for ozone therapy in dental practice (Nogales et al., 2008). Despite its potential benefits, the application of ozone therapy in dentistry is limited due to possible side effects (Naik et al., 2016). Therefore, dental practitioners need to have a proper understanding of ozone therapy and its proper usage to provide better patient care and reduce the time and cost of treatment (Naik et al., 2016).

In conclusion, ozone therapy has shown potential as an adjunct treatment in dental practice. It has antimicrobial, disinfectant, anti-inflammatory, and healing properties that make it a promising option for various dental conditions. However, further research is needed to establish safe and well-defined parameters for its use in dental practice. Dental practitioners should stay updated with the latest evidence and guidelines regarding ozone therapy to provide optimal care to their patients.

REFERENCES

AL-Omiri, M., Nazeh, A., Kielbassa, A. (2018). Randomized Controlled Clinical Trial On Bleaching Sensitivity and Whitening Efficacy Of Hydrogen Peroxide Versus Combinations Of Hydrogen Peroxide And Ozone. Sci Rep, 1(8). https://doi.org/10.1038/s41598-018-20878-0 AlMogbel, A., Albarrak, M., AlNumair, S. (2023). Ozone Therapy In the Management And Prevention Of Caries. Cureus. https://doi.org/10.7759/cureus.37510 Anzolin, A., Silveira-Kaross, N., Bertol, C. (2020). Ozonated Oil In Wound Healing: What Has Already Been Proven?. Med Gas Res, 1(10), 54. https://doi.org/10.4103/2045-9912.279985 Barczyk, I., Masłyk, D., Walczuk, N., Kijak, K., Skomro, P., Gronwald, H., … & Lietz-Kijak, D. (2023). Potential Clinical Applications Of Ozone Therapy In Dental Specialties—a Literature Review, Supported By Own Observations. IJERPH, 3(20), 2048. https://doi.org/10.3390/ijerph20032048 Baysan, A., Whiley, R., Lynch, E. (2000). Antimicrobial Effect Of a Novel Ozone– Generating Device On Micro–organisms Associated With Primary Root Carious Lesions In Vitro. Caries Res, 6(34), 498-501. https://doi.org/10.1159/000016630 Butera, A., Pascadopoli, M., Gallo, S., Martínez, C., Val, J., Parisi, L., … & Scribante, A. (2023). Ozonized Hydrogels Vs. 1% Chlorhexidine Gel For the Clinical And Domiciliary Management Of Peri-implant Mucositis: A Randomized Clinical Trial. JCM, 4(12), 1464. https://doi.org/10.3390/jcm12041464 Grillo, R., Campos, F., Jodas, C. (2022). Alternative Approach To Treating Dark Circles: a Case Report. J of Cosmetic Dermatology, 12(21), 6909-6912. https://doi.org/10.1111/jocd.15349 Jain, M., Mishra, R., Mishra, R., Ghritlahare, H., Pathak, A., Shukla, S. (2021). Ozone Therapy-new Innovation In Dentistry. ijhs, 444-452. https://doi.org/10.53730/ijhs.v5ns2.5866 Kazancioglu, H., Ezirganli, S., Demirtaş, N. (2013). Comparison Of the Influence Of Ozone And Laser Therapies On Pain, Swelling, And Trismus Following Impacted Third-molar Surgery. Lasers Med Sci, 4(29), 1313-1319. https://doi.org/10.1007/s10103-013-1300-y Marconcini, S. (2023). The Effect Of Ozonized Water Irrigation In the Circuits Of Professional Ultrasonic Scalers For Causal Therapy Of Stage I Periodontitis: A Randomized Clinical Study. J Dent Hyg Sci, 1(23), 13-19. https://doi.org/10.17135/jdhs.2023.23.1.13 Meira, A., Santos, A., Lima, C., Milhomens, F., Araujo-Silva, G., Cardoso, M., … & Filho, J. (2022). Use and Applicability Of Ozone Therapy In Clinical Practice In Dentistry: An Integrative Review. Int. J. Odontostomat., 4(16), 468-474. https://doi.org/10.4067/s0718-381×2022000400468 Mithun, D., Moses, J., Sharanya, N. (2022). Ozone Therapy In Management and Prevention Of Dental Caries- A Review. IJPedoR, 2(7), 25-29. https://doi.org/10.56501/intjpedorehab.v7i2.579 Mostafa, B., Zakaria, M. (2018). Evaluation Of Combined Topical Ozone and Steroid Therapy In Management Of Oral Lichen Planus. Open Access Maced J Med Sci, 5(6), 879-884. https://doi.org/10.3889/oamjms.2018.219 Mousa, H. (2023). Clinical and Radiographic Evaluation Of Ozone Therapy In Odontectomy Of Impacted Mandibular Third Molar. Int. J. Appl. Dent. Sci., 1(9), 307-311. https://doi.org/10.22271/oral.2023.v9.i1e.1697 Nagayoshi, M., Fukuizumi, T., Kitamura, C., Yano, J., Terashita, M., Nishihara, T. (2004). Efficacy Of Ozone On Survival and Permeability Of Oral Microorganisms. Oral Microbiol Immunol, 4(19), 240-246. https://doi.org/10.1111/j.1399-302x.2004.00146.x Naik, S., Rajeshwari, K., Kohli, S., Zohabhasan, S., Bhatia, S. (2016). Ozone- a Biological Therapy In Dentistry- Reality Or Myth?????. TODENTJ, 1(10), 196-206. https://doi.org/10.2174/1874210601610010196 Nogales, C., Ferrari, P., Kantorovich, E., Lage-Marques, J. (2008). Ozone Therapy In Medicine and Dentistry. The Journal of Contemporary Dental Practice, 4(9), 75-84. https://doi.org/10.5005/jcdp-9-4-75 Roth, A., Maruthamuthu, M., Nejati, S., Krishnakumar, A., Selvamani, V., Sedaghat, S., … & Rahimi, R. (2022). Wearable Adjunct Ozone and Antibiotic Therapy System For Treatment Of Gram-negative Dermal Bacterial Infection. Sci Rep, 1(12). https://doi.org/10.1038/s41598-022-17495-3 Rusdy, H. (2023). The Disinfection Effectiveness Of Ozone Water and 4.8% Chloroxylenol Against The Number Of Bacterial Colonies In Dental Extraction Instruments At The Usu Dental And Oral Hospital In October-december 2022. F1000Res, (12), 726. https://doi.org/10.12688/f1000research.132941.1 Saini, R. (2011). Ozone Therapy In Dentistry: a Strategic Review. J Nat Sc Biol Med, 2(2), 151. https://doi.org/10.4103/0976-9668.92318 Sen, S., Sen, S. (2020). Ozone Therapy a New Vista In Dentistry: Integrated Review. Med Gas Res, 4(10), 189. https://doi.org/10.4103/2045-9912.304226 Silva, E., Morais, B., Vivacqua, F. (2022). Association Of Ozoniotherapy To Endodontics, Aiming At the Tissue Repair Of Periapical Lesions – A Literature Review / Associação Da Ozonioterapia à Endodontia, Visando O Reparo Tecidual De Lesões Periapicais – Uma Revisão De Literatura. BASR, 4(6), 1292-1304. https://doi.org/10.34115/basrv6n4-005 Srikanth, A., Sathish, M., Harsha, A. (2013). Application Of Ozone In the Treatment Of Periodontal Disease. J Pharm Bioall Sci, 5(5), 89. https://doi.org/10.4103/0975-7406.113304 Suh, Y., Patel, S., Kaitlyn, R., Gandhi, J., Joshi, G., Smith, N., … & Khan, S. (2019). Clinical Utility Of Ozone Therapy In Dental and Oral Medicine. Med Gas Res, 3(9), 163. https://doi.org/10.4103/2045-9912.266997 Özbay, İ., Ital, I., Kucur, C., Akcılar, R., Deger, A., Aktas, S., … & Oghan, F. (2017). Effects Of Ozone Therapy On Facial Nerve Regeneration. Brazilian Journal of Otorhinolaryngology, 2(83), 168-175. https://doi.org/10.1016/j.bjorl.2016.02.009